2 edition of Characterization of a novel surface protein of endothelial cells and pre-B leukemic cells. found in the catalog.
Characterization of a novel surface protein of endothelial cells and pre-B leukemic cells.
Written in English
|The Physical Object|
|Number of Pages||149|
Characterization of the cell surface heterodimer VLA-4 and related peptides Article (PDF Available) in Journal of Biological Chemistry (24) August with 70 Reads. The purpose of this study was to isolate endothelial cells from different organs of porcine fetuses and to examine the binding of endothelial markers including lectins. Endothelial cells were isolated from the aorta, cerebral cortex, myocardium, ovary and by:
In situ patch clamp characterization of K channels in the luminal surface of the endothelial cell layer in rat superior mesenteric artery Characterization of a novel surface protein of. Previous studies showed that Fab fragments (Fab) of the antibody raised against the surface of V79 cells inhibit the Ca 2+-independent aggregation of V79 cells, and that this inhibitory effect is neutralized by the addition of some proteinous substance released from the surface of V79 cells treated with % trypsin. In the present study, we.
Precursor B-cell acute lymphoblastic leukemia (pre-B ALL) is the most prevalent malignancy of childhood surface structures that selectively identify leukemia cells Cited by: 1. present on the surface of endothelial cells. Although there was no evidence for an in vitro or in vivo regulated storage and release pathway for PrPC, in vitro experiments sugges-ted that both cell types could constitutively release PrPC, both as a soluble protein and microparticle bound. In vivo measurements of PrPC in patients with widely Cited by:
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The antigenic structures recognized by anti-endothelial cell antibodies (AECA) in sera from 10 Wegener's granulomatosis (WG) and 12 systemic lupus erythematosus (SLE) patients with signs of vasculitis were characterized by immunoprecipitation of selectively radiolabeled surface membrane proteins from human umbilical vein endothelial by: Leukocyte-endothelial cell interactions are centra to the normal re-circulation of lymphocytes and to pathological situations such as inflammation.
In vitro studies aimed at dissecting molecular processes underlying these interactions would benefit greatly from a stable, reproducible, homogeneous source of human endothelial : J. Cartwright, G. St J. Whitley, A. Johnstone. Ferrero E, Malavasi F. Human CD38, a leukocyte receptor and ectoenzyme, is a member of a novel eukaryotic gene family of nicotinamide adenine dinucleotide+-converting enzymes: extensive structural homology with the genes for murine bone marrow stromal cell antigen 1 and aplysian ADP-ribosyl by: CD34 positive leukemic cells in BM of pre B cell ALL patient detected by FITC-conjugated 27H2 and HPCA-2 were identi-fied with similar sensitivity.
Conclusion: A novel murine mAb recognizing class III epitope of human CD34 with high affin-ity, which is useful. Endothelial cells (ECs) govern tissue development and regener-ation by signaling molecules ontheir cell surfaceand therelease of factors, such as cytokines and extracellular matrix proteins.
This angiocrine function of the endothelium drives tissue devel-opment. These angiogenic cells have properties of embryonal angioblasts and were termed endothelial progenitor cells (EPCs).
In general, three surface markers (CD, CD34 and the vascular endothelial growth factor receptor‐2) characterize the early functional angioblast, located Cited by: The lymphatic microvasculature is uniquely adapted for the continuous removal of interstitial fluid and proteins and is an important entry point for leukocytes and tumor cells.
Specialized functions of lymphatics suggest differences in the molecular composition of the lymphatic and blood vascular endothelium.
However, the extent to which the two cell types differ is still unclear, and few Cited by: Human endothelial cells proliferation inhibition assay. Human endothelial cells under subconfluent conditions (5 × 10 3 cells/well) were plated onto well culture plates in μl of EBM-2 medium without FBS, and incubated at 37 °C in a CO 2 incubator overnight.
Various amounts (,1 and 2 μg/ml) of ARap, SN6h monoclonal Cited by: Endothelial progenitor cells (EPCs) are increasingly becoming a major focus of regenerative medicine research and practice.
The present study was undertaken to establish an appropriate procedure for isolation and characterization of EPCs from Rhesus monkeys for regenerative medicine research.
Selective CD34+ and nonselective mononuclear EPCs were isolated from bone Cited by: 6. The human signal-regulatory protein (SIRP), described as a stem cell marker very recently, is a serious candidate protein for distinction between hematopoietic progenitor cells Cited by: Ang-2 expressed in CD7(+)AML cells is not considered to act in an autocrine fashion, but to work on endothelial cells to ‘feed’ leukemic by: Immunologic Properties of Endothelial Cells Human EC contain ABO blood group and HLA-A,B antigens.1' In contrast, only a small per- centage of unstimulated EC contain Ia antigens However, when human EC are stimulated with ~/-in- terferon or by activated T cells, Ia antigens are in- duced on the surface of all EC exposed to the stim- ulus Cited by: Preincubation of staphylococci with the purified protein significantly (P less than ) reduced staphylococcal binding to cultured endothelial cells.
The capacity of S. aureus to colonize and invade endovascular surfaces may in part be a consequence of staphylococcal interaction with this endothelial cell membrane by: Increasing evidence links blood coagulation proteins with the regulation of acute and chronic inflammatory disease.
Of particular interest are vitamin K-dependent proteases, which are generated as a hemostatic response to vascular injury, but can also initiate signal transduction via interactions with vascular receptors.
The endothelial cell protein C receptor (EPCR) is a multi-ligand Cited by: Since leukemic stem cells (LSCs) or cancer stem cells (CSCs) were found in acute myeloid leukemia (AML) inextensive studies have been contributed to identification and characterization of such cell populations in various tissues.
LSCs are now generally recognized as a heterogeneous cell population that possesses the capacities of self-renewal, proliferation and Cited by: The protein PrPc is expressed also in non-neural cells, such as immune cells, epithelial and endothelial cells (Cazaubon et al., ).
Furthermore estrogens, and in particular E 2, play a pivotal role in a large number of physiological processes, including those concerning the circulatory by: 1. Thrombin binds to thrombomodulin, a protein expressed in endothelial cells, and activates protein C. The endothelial protein C receptor (EPCR) is also important in this process.
The synthesis of tissue plasminogen activator (t-PA) by endothelial cells promotes conversion of plasminogen to plasmin, which triggers fibrinolysis.
The characterization of circulating endothelial progenitor cells (EPCs) is fundamental to any study related to angiogenesis. Unfortunately, current literature lacks consistency in the definition of EPC subsets due to variations in isolation strategies and inconsistencies in the use of lineage by: 8.
Characterization of acute lymphoblastic leukemia progenitor cells Article (PDF Available) in Blood (9) December with Reads How we measure 'reads'. Primary mouse leukocytes from spleen, thymus, lymph node, and peritoneum were activated in vitro, and CD4+ and CD8+ T cells as well as B cells expressed surface LT ligand but not the LT beta R.
Over recent years, interest in endothelial cell biology has increased dramatically with our ability to grow and study endothelial cellsin vitro. While large veins and arteries remain a quick and convenient source of endothelial cells, the great morphological, biochemical and functional heterogeneity that endothelial cells express has necessitated the development of techniques to Cited by: MSCs are stem cells for the entire mesenchymal lineage, producing bone, cartilage, and fat cells, as well as various fibroblast-like stromal cells with essential scaffolding roles in depositing and shaping ECM by: b, Necroptotic endothelial-cell death is induced by amyloid precursor protein (APP) on the tumour surface, which interacts with the death receptor 6 protein (DR6) on endothelial cells.
Tumour-cell Cited by: 1.